Article
Construction and Analysis of GmFAD2-1A and GmFAD2-2A Soybean Fatty Acid Desaturase Mutants Based on CRISPR/Cas9 Technology.
International journal of molecular sciences - 7 Feb 2020
Wu Nan, Lu Qiang, Wang Piwu, Zhang Qi, Zhang Jun, Qu Jing, Wang Nan
Abstract excerpt
The soybean fatty acid desaturase family is composed of seven genes, but the function of each gene has not been reported. Bioinformatics was used to analyse the structure of genes in this family, as well as the correlation between Δ12-fatty acid desaturase II (FAD2) expression and oleic acid content on different days after flowering of soybean. In the present study, CRISPR/Cas9 technology was used to construct...
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