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Article

hTERT-Driven Immortalization of RDEB Fibroblast and Keratinocyte Cell Lines Followed by Cre-Mediated Transgene Elimination.

International journal of molecular sciences - 7 Apr 2021

Evtushenko Nadezhda A, Beilin Arkadii K, Dashinimaev Erdem B, Ziganshin Rustam H, Kosykh Anastasiya V, Perfilov Maxim M, Rippa Alexandra L, Alpeeva Elena V, Vasiliev Andrey V, Vorotelyak Ekaterina A, Gurskaya Nadya G

Abstract excerpt

The recessive form of dystrophic epidermolysis bullosa (RDEB) is a crippling disease caused by impairments in the junctions of the dermis and the basement membrane of the epidermis. Using ectopic expression of hTERT/hTERT + BMI-1 in primary cells, we developed expansible cultures of RDEB fibroblasts and keratinocytes. We showed that they display the properties of their founders, including morphology, contraction...

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Identifiers

PMID: 33916959
DOI: 10.3390/ijms22083809
PMCID: PMC8067634

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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