Article
Injection of genetically engineered fibroblasts corrects regenerated human epidermolysis bullosa skin tissue
15 Jan 2003
Abstract excerpt
MethodsRDEB cells.Fibroblasts from four unrelated COL7A1 mutant type VII collagen-deficient RDEB patients (3) fulfilling clinical, immunohistological, ultrastructural, and genetic criteria for the disease (11) were grown as described (1).Integrase-based stable integration of the type VII collagen expression plasmid, pCOL7A1, was performed by cotransfecting fibroblasts with a φC31 integrase-encoding plasmid and...
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