Article
Three isolation techniques for primary culture of human osteoblast-like cells: a comparison.
Acta orthopaedica Scandinavica - 1 Aug 1999
Jonsson K B, Frost A, Nilsson O, Ljunghall S, Ljunggren O
Abstract excerpt
The culture of osteoblast-like cells of human origin has become an important experimental model in bone biology. We report here a comparison and evaluation of three of the most widely used systems available today: bone marrow stroma cell cultures (BMSC), human osteoblast explant cultures (hOB) and osteoblast explant cultures from collagenase-treated bone (hOBcol). Cultures from 16 bone specimens obtained from...
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