Article
Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases.
Nature communications - 20 Apr 2021
Zhang Minjie, Li Kongpan, Bai Jianhui, Velema Willem A, Yu Chengqing, van Damme Ryan, Lee Wilson H, Corpuz Maia L, Chen Jian-Fu, Lu Zhipeng
Abstract excerpt
Direct determination of RNA structures and interactions in living cells is critical for understanding their functions in normal physiology and disease states. Here, we present PARIS2, a dramatically improved method for RNA duplex determination in vivo with >4000-fold higher efficiency than previous methods. PARIS2 captures ribosome binding sites on mRNAs, reporting translation status on a transcriptome scale....
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