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A high-efficient strategy for combinatorial engineering paralogous gene family: A case study on histidine kinases in Clostridium.

Biotechnology and bioengineering - 1 Jul 2021

Zhu Chao, Du Guangqing, Zhang Jie, Xue Chuang

Abstract excerpt

Microorganisms harbor bulks of functionally similar or undefined genes, which belong to paralogous gene family. There is a necessity of exploring combinatorial or interactive functions of these genes, but conventional loss-of-function strategy with one-by-one rounds suffers extremely low efficiency for generating mutant libraries with all gene permutations. Here, taking histidine kinases (HKs) in Clostridium...

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PMID: 33871069
DOI: 10.1002/bit.27796

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