Article
CRISPR/Cas9-mediated mutagenesis reveals the roles of calaxin in gastropod larval cilia.
Gene - 30 Jun 2021
Huan Pin, Cui Menglu, Wang Qian, Liu Baozhong
Abstract excerpt
Obtaining detectable knockout phenotypes in the G0 generation is essential for gene function studies. Although CRISPR/Cas9-mediated gene editing has been employed to knock out molluscan genes, detectable phenotypes in the G0 generation have not been reported in these animals. In this study, we determined the knockout phenotype of a cilium-related gene, calaxin, using CRISPR/Cas9 technology in the gastropod...
Topics
- Animals
- CRISPR-Cas Systems
- Calcium-Binding Proteins
- Cilia
- Gastropoda
- Gene Knockout Techniques
- Membrane Proteins
- Mutagenesis
- Polymorphism, Single Nucleotide