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How to characterize a strain? Clonal heterogeneity in industrial Saccharomyces influences both phenotypes and heterogeneity in phenotypes.

Yeast (Chichester, England) - 1 Aug 2021

Rácz Hanna Viktória, Mukhtar Fezan, Imre Alexandra, Rádai Zoltán, Gombert Andreas Károly, Rátonyi Tamás, Nagy János, Pócsi István, Pfliegler Walter P

Abstract excerpt

Populations of microbes are constantly evolving heterogeneity that selection acts upon, yet heterogeneity is nontrivial to assess methodologically. The necessary practice of isolating single-cell colonies and thus subclone lineages for establishing, transferring, and using a strain results in single-cell bottlenecks with a generally neglected effect on the characteristics of the strain itself. Here, we present...

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PMID: 33844327
DOI: 10.1002/yea.3562

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