Article
Development of biotinylated and magnetic bead-immobilized enzymes for efficient glyco-engineering and isolation of antibodies.
Bioorganic chemistry - 1 Jul 2021
Chuang Hong-Yang, Huang Chiu-Chen, Hung Ting-Chun, Huang Lin-Ya, Chiu Chih-Wei, Chu Kuo-Ching, Liao Jung-Yu, You Tsai-Hong, Wu Chung-Yi, Chao Ping, Shivatare Sachin S, Zeng Yi-Fang, Tsai Charng-Sheng, Lin Nan-Horng, Wu Chung-Yi
Abstract excerpt
The chemoenzymatic remodeled monoclonal antidodies with well-defined glycan structure at the Fc domain display improved biological activities, such as ADCC and ADCP, and are more likely to yield a better safety profile by eliminating the non-human glycans derived from CHO cell culture. We covalently immobilize wild type endoglycosidase S (EndoS), fucosidase, and EndoS2 mutant on magnetic beads through a linker to...
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