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Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: in vitro assay system for plant cPNGase activity.

Bioscience, biotechnology, and biochemistry - 25 May 2021

Shirai Sahoko, Uemura Ryota, Maeda Megumi, Kajiura Hiroyuki, Misaki Ryo, Fujiyama Kazuhito, Kimura Yoshinobu

Abstract excerpt

Cytosolic peptide:N-glycanase (cPNGase), which occurs ubiquitously in eukaryotic cells, is involved in the de-N-glycosylation of misfolded glycoproteins in the protein quality control system. In this study, we aimed to provide direct evidence of plant cPNGase activity against a denatured glycoprotein using a crude extract prepared from a mutant line of Arabidopsis thaliana lacking 2 acidic PNGase genes.

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PMID: 33724383
DOI: 10.1093/bbb/zbab047

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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