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Genome engineering of Nannochloropsis with hundred-kilobase fragment deletions by Cas9 cleavages.

The Plant journal : for cell and molecular biology - 1 May 2021

Wang Qintao, Gong Yanhai, He Yuehui, Xin Yi, Lv Nana, Du Xuefeng, Li Yun, Jeong Byeong-Ryool, Xu Jian

Abstract excerpt

Industrial microalgae are promising photosynthetic cell factories, yet tools for large-scale targeted genome engineering are limited. Here for the model industrial oleaginous microalga Nannochloropsis oceanica, we established a method to precisely and serially delete large genome fragments of ~100 kb from its 30.01 Mb nuclear genome. We started by identifying the 'non-essential' chromosomal regions (i.e. low...

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CRISPR-Cas Systems
Genetic Engineering
Genome
Microalgae
Phenotype

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PMID: 33719095
DOI: 10.1111/tpj.15227

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