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Article

High-throughput Raman-activated cell sorting of microalgal genome-wide edited library revealed a regulatory pathway for carotenoid synthesis.

Nature communications - 12 Jun 2026

Wang Qintao, Gong Yanhai, Wang Lianhong, Lv Nana, Du Xuefeng, Zhang Jiashun, Xin Yi, Nikoloski Zoran, Li-Beisson Yonghua, Sun Luyang, Ma Bo, Wang Xixian, Xu Jian

Abstract excerpt

Functional genomics have been hampered by the paucity of efficient methods that connect genotype and metabolic phenotype at single-cell resolution. Using the industrial microalga Nannochloropsis oceanica as a model, we introduced a platform that comprises a genome-wide single-gene-edited mutant library and high-throughput Raman-activated cell sorting (RACS). The CRISPR/Cas-generated library consisted of 3567...

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Identifiers

PMID: 42277013
DOI: 10.1038/s41467-026-74304-5
PMCID: PMC13473490

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