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Article

Analysis of a small outbreak of Shiga toxin-producing Escherichia coli O157:H7 using long-read sequencing.

Microbial genomics - 1 Mar 2021

Greig David R, Jenkins Claire, Gharbia Saheer E, Dallman Timothy J

Abstract excerpt

Compared to short-read sequencing data, long-read sequencing facilitates single contiguous de novo assemblies and characterization of the prophage region of the genome. Here, we describe our methodological approach to using Oxford Nanopore Technology (ONT) sequencing data to quantify genetic relatedness and to look for microevolutionary events in the core and accessory genomes to assess the within-outbreak...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33683192
DOI: 10.1099/mgen.0.000545
PMCID: PMC8190617

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