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Article

Adaptive Laboratory Evolution Restores Solvent Tolerance in Plasmid-Cured Pseudomonas putida S12: a Molecular Analysis.

Applied and environmental microbiology - 13 Apr 2021

Kusumawardhani Hadiastri, Furtwängler Benjamin, Blommestijn Matthijs, Kaltenytė Adelė, van der Poel Jaap, Kolk Jan, Hosseini Rohola, de Winde Johannes H

Abstract excerpt

Pseudomonas putida S12 is inherently solvent tolerant and constitutes a promising platform for biobased production of aromatic compounds and biopolymers. The megaplasmid pTTS12 of P. putida S12 carries several gene clusters involved in solvent tolerance, and the removal of this megaplasmid caused a significant reduction in solvent tolerance. In this study, we succeeded in restoring solvent tolerance in...

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PMID: 33674430
DOI: 10.1128/AEM.00041-21
PMCID: PMC8091024

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