Rapid 3D phenotypic analysis of neurons and organoids using data-driven cell segmentation-free machine learning.

Abstract excerpt

Phenotypic profiling of large three-dimensional microscopy data sets has not been widely adopted due to the challenges posed by cell segmentation and feature selection. The computational demands of automated processing further limit analysis of hard-to-segment images such as of neurons and organoids. Here we describe a comprehensive shallow-learning framework for automated quantitative phenotyping of...

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