Article
A rapid and cost-effective multiplex ARMS-PCR method for the simultaneous genotyping of the circulating SARS-CoV-2 phylogenetic clades.
Journal of medical virology - 1 May 2021
Islam Mohammad Tanvir, Alam Asm Rubayet Ul, Sakib Najmuj, Hasan Mohammad Shazid, Chakrovarty Tanay, Tawyabur Mohammad, Islam Ovinu Kibria, Al-Emran Hassan M, Jahid Mohammad Iqbal Kabir, Anwar Hossain Mohammad
Abstract excerpt
Tracing the globally circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) phylogenetic clades by high-throughput sequencing is costly, time-consuming, and labor-intensive. We here propose a rapid, simple, and cost-effective amplification refractory mutation system (ARMS)-based multiplex reverse-transcription polymerase chain reaction (PCR) assay to identify six distinct phylogenetic clades: S,...
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