Article
Dedicated transcriptomics combined with power analysis lead to functional understanding of genes with weak phenotypic changes in knockout lines.
PLoS computational biology - 1 Nov 2020
Xie Chen, Bekpen Cemalettin, Künzel Sven, Keshavarz Maryam, Krebs-Wheaton Rebecca, Skrabar Neva, Ullrich Kristian K, Zhang Wenyu, Tautz Diethard
Abstract excerpt
Systematic knockout studies in mice have shown that a large fraction of the gene replacements show no lethal or other overt phenotypes. This has led to the development of more refined analysis schemes, including physiological, behavioral, developmental and cytological tests. However, transcriptomic analyses have not yet been systematically evaluated for non-lethal knockouts. We conducted a power analysis to...
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