Article
Amplicon-Based, Next-Generation Sequencing Approaches to Characterize Single Nucleotide Polymorphisms of Orthohantavirus Species.
Frontiers in cellular and infection microbiology - 1 Jan 2020
Taylor Mariah K, Williams Evan P, Wongsurawat Thidathip, Jenjaroenpun Piroon, Nookaew Intawat, Jonsson Colleen B
Abstract excerpt
Whole-genome sequencing (WGS) of viruses from patient or environmental samples can provide tremendous insight into the epidemiology, drug resistance or evolution of a virus. However, we face two common hurdles in obtaining robust sequence information; the low copy number of viral genomes in specimens and the error introduced by WGS techniques. To optimize detection and minimize error in WGS of hantaviruses, we...
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