Article

Optimized workflow for high-throughput whole genome surveillance of Influenza A virus

2024-10-25

Abstract excerpt

<title>Abstract</title> <p>Whole genome sequencing (WGS) is crucial for studying influenza A virus (IAV) genomic diversity in various host species to mitigate its impact on human and animal health. While the multisegment RT-PCR (mRT-PCR) efficiently amplifies all genomic segments in a single reaction, its sensitivity for larger segments is suboptimal. To improve WGS sensitivity, we optimized the mRT-PCR protocol...

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Literature Corpus work: 05958aa3-1a35-5632-9d36-df9c20e783ac
DOI: 10.21203/rs.3.rs-5216720/v1

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