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Optimized high-throughput whole-genome sequencing workflow for surveillance of influenza A virus

26 Sept 2025

Abstract excerpt

Whole-genome sequencing (WGS) is essential for monitoring the genetic diversity of influenza A virus (IAV) across host species. We optimized a multisegment RT-PCR (mRT-PCR) protocol to enhance amplification of all eight IAV segments using modified RT and PCR conditions. Additionally, we introduced a dual-barcoding approach for the Oxford Nanopore platform, enabling high-throughput multiplexing without...

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PMID: 41013580
DOI: 10.1186/s13073-025-01512-x

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