Article
Site-specific dynamic nuclear polarization in a Gd(III)-labeled protein.
Physical chemistry chemical physics : PCCP - 18 Nov 2020
Heiliger Jörg, Matzel Tobias, Çetiner Erhan Can, Schwalbe Harald, Kuenze Georg, Corzilius Björn
Abstract excerpt
Dynamic nuclear polarization (DNP) of a biomolecule tagged with a polarizing agent has the potential to not only increase NMR sensitivity but also to provide specificity towards the tagging site. Although the general concept has been often discussed, the observation of true site-specific DNP and its dependence on the electron-nuclear distance has been elusive. Here, we demonstrate site-specific DNP in a uniformly...
Topics
- Gadolinium
- Isotope Labeling
- Mutation
- Nuclear Magnetic Resonance, Biomolecular
- Recombinant Proteins
- Ubiquitin
