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Article

Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli.

eLife - 21 Oct 2020

Flamholz Avi I, Dugan Eli, Blikstad Cecilia, Gleizer Shmuel, Ben-Nissan Roee, Amram Shira, Antonovsky Niv, Ravishankar Sumedha, Noor Elad, Bar-Even Arren, Milo Ron, Savage David F

Abstract excerpt

Many photosynthetic organisms employ a CO2 concentrating mechanism (CCM) to increase the rate of CO2 fixation via the Calvin cycle. CCMs catalyze ≈50% of global photosynthesis, yet it remains unclear which genes and proteins are required to produce this complex adaptation. We describe the construction of a functional CCM in a non-native host, achieved by expressing genes from an autotrophic bacterium in an...

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Identifiers

PMID: 33084575
DOI: 10.7554/eLife.59882
PMCID: PMC7714395

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