Article
Fgf10-CRISPR mosaic mutants demonstrate the gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung.
PloS one - 1 Jan 2020
Habuta Munenori, Yasue Akihiro, Suzuki Ken-Ichi T, Fujita Hirofumi, Sato Keita, Kono Hitomi, Takayama Ayuko, Bando Tetsuya, Miyaishi Satoru, Oyadomari Seiichi, Tanaka Eiji, Ohuchi Hideyo
Abstract excerpt
CRISPR/Cas9-mediated gene editing often generates founder generation (F0) mice that exhibit somatic mosaicism in the targeted gene(s). It has been known that Fibroblast growth factor 10 (Fgf10)-null mice exhibit limbless and lungless phenotypes, while intermediate limb phenotypes (variable defective limbs) are observed in the Fgf10-CRISPR F0 mice. However, how the lung phenotype in the Fgf10-mosaic mutants is...
Topics
- Alveolar Epithelial Cells
- Animals
- Clustered Regularly Interspaced Short Palindromic Repeats
- Disease Models, Animal
- Embryo, Mammalian
- Fibroblast Growth Factor 10
- Gene Dosage
- Gene Editing
- Genotype