Article
Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele.
Nature communications - 6 Oct 2020
De Meester Barbara, Madariaga Calderón Barbara, de Vries Lisanne, Pollier Jacob, Goeminne Geert, Van Doorsselaere Jan, Chen Mingjie, Ralph John, Vanholme Ruben, Boerjan Wout
Abstract excerpt
Lignin causes lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Engineered low-lignin plants have reduced recalcitrance but often exhibit yield penalties, offsetting their gains in fermentable sugar yield. Here, CRISPR/Cas9-generated CCR2(-/*) line 12 poplars have one knockout CCR2 allele while the other contains a 3-bp deletion, resulting in a 114I115A-to-114T conversion in the corresponding...
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