Article
Breeding with rare defective alleles (BRDA): a natural Populus nigra HCT mutant with modified lignin as a case study.
The New phytologist - 1 May 2013
Vanholme Bartel, Cesarino Igor, Goeminne Geert, Kim Hoon, Marroni Fabio, Van Acker Rebecca, Vanholme Ruben, Morreel Kris, Ivens Bart, Pinosio Sara, Morgante Michele, Ralph John, Bastien Catherine, Boerjan Wout
Abstract excerpt
Next-generation (NG) sequencing in a natural population of Populus nigra revealed a mutant with a premature stop codon in the gene encoding hydroxycinnamoyl-CoA : shikimate hydroxycinnamoyl transferase1 (HCT1), an essential enzyme in lignin biosynthesis. The lignin composition of P. nigra trees homozygous for the defective allele was compared with that of heterozygous trees and trees without the defective allele....
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