TALEN-mediated targeted mutagenesis of more than 100 COMT copies/alleles in highly polyploid sugarcane improves saccharification efficiency without compromising biomass yield.

Abstract excerpt

Sugarcane is the world's most efficient feedstock for commercial production of bioethanol due to its superior biomass production and accumulation of sucrose in stems. Integrating first- and second-generation ethanol conversion processes will enhance the biofuel yield per unit area by utilizing both sucrose and cell wall-bound sugars for fermentation. RNAi suppression of the lignin biosynthetic gene caffeic acid...

Topics

• Biomass
• Cell Wall
• Gene Dosage
• Gene Expression Regulation, Plant
• Glucose
• Lignin
• Methyltransferases
• Mutagenesis
• Mutation Rate
• Plants, Genetically Modified