Article
Generation of a tdTomato-GAD67 reporter human epilepsia mutation induced pluripotent stem cell line, USTCi001-A-2, using CRISPR/Cas9 editing.
Stem cell research - 1 Oct 2020
Zhao Huifang, He Lang, Huang Hualin, Li Shuai, Cheng Na, Tang Feng, Han Xiaobo, Lin Zuoxian, Huang Rongqi, Zhou Peng, Deng Sihao, Huang Jufang, Li Zhiyuan
Abstract excerpt
Dravet syndrome is an epileptic encephalopathy largely due to haploinsufficiency of the voltage-gated sodium channel Nav1.1 that is expressed primarily in GABAergic neurons. In order to distinguish the different subtypes, we used gene editing to introduce tdTomato gene into the genome of iPSCs to label the GABAergic neurons in the differentiated neuronal networks. The gene-edited cell line demonstrates normal...
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