Article
A tailed PCR procedure for cost-effective, two-order multiplex sequencing of candidate genes in polyploid plants.
Plant biotechnology journal - 1 Aug 2012
Gholami Mahmood, Bekele Wubishet A, Schondelmaier Joerg, Snowdon Rod J
Abstract excerpt
Complex polyploid crop genomes can be recalcitrant towards conventional DNA sequencing approaches for allele mining in candidate genes for valuable traits. In the past, this has greatly complicated the transfer of knowledge on promising candidate genes from model plants to even closely related polyploid crops. Next-generation sequencing offers diverse solutions to overcome such difficulties. Here, we present a...
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