Article
Detection of feline coronavirus RNA, spike gene mutations, and feline coronavirus antigen in macrophages in aqueous humor of cats in the diagnosis of feline infectious peritonitis.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Jul 2020
Sangl Laura, Felten Sandra, Matiasek Kaspar, Dörfelt Stefanie, Bergmann Michele, Balzer Hans-Jörg, Pantchev Nikola, Leutenegger Christian, Hartmann Katrin
Abstract excerpt
Uveitis is common in cats, and is often a feature of feline infectious peritonitis (FIP). We evaluated 3 tools for detection of feline coronavirus (FCoV) in aqueous humor: 1) a 7b gene reverse-transcription real-time PCR (7b-RT-rtPCR) assay to detect FCoV RNA, 2) a spike gene mutation RT-rtPCR (S-RT-rtPCR) assay to detect 2 point mutations in the spike gene of FCoV in cats positive by 7b-RT-rtPCR, and 3)...
Topics
- Animals
- Aqueous Humor
- Case-Control Studies
- Cats
- Coronavirus Infections
- Coronavirus, Feline
- Feline Infectious Peritonitis
- Immunohistochemistry
- Macrophages