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Rapid detection and quantification of plasmid‐mediated colistin resistance genes ( mcr‐1 to mcr‐5 ) by real‐time PCR in bacterial and environmental samples

8 Jun 2020

Abstract excerpt

Aim The aim of the study was to validate a rapid method to detect and quantify colistin resistance genes (mcr-1 to mcr-5) by real-time polymerase chain reaction (RT-PCR) in diverse matrices. Methods and results The detection limit of two newly designed SYBR Green real-time PCR assays for mcr-4 and mcr-5 and of previously published protocols for mcr-1 to mcr-3 was assessed using serial dilutions of reference...

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PMID: 32510809
DOI: 10.1111/jam.14738

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