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Article

Characterization of an anti-FLAG antibody binding protein in V. cholerae.

Biochemical and biophysical research communications - 30 Jul 2020

Shin Jung-Ho, Lanz Michael, Smolka Marcus B, Dörr Tobias

Abstract excerpt

FLAG-tags are commonly used for protein abundance measurements and for identification of protein-protein interactions in living cells. We have observed that the cholera pathogen Vibrio cholerae encodes a FLAG-antibody-reactive protein and identified this protein as an outer membrane porin, Porin4, which contains a sequence very similar to the 3xFLAG epitope tag. We have demonstrated the binding affinity of the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32505345
DOI: 10.1016/j.bbrc.2020.05.169
PMCID: PMC7357423

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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