Article
Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage.
PloS one - 1 Jan 2020
Pandya Priyanka, Sayers Robert O, Ting Joey P, Morshedian Shaghayegh, Torres Carina, Cudal Justine S, Zhang Kai, Fitchett Jonathan R, Zhang Qing, Zhang Feiyu F, Wang Jing, Durbin Jim D, Carrillo Juan J, Espada Alfonso, Broughton Howard, Qian Yuewei, Afshar Sepideh
Abstract excerpt
Hundreds of target specific peptides are routinely discovered by peptide display platforms. However, due to the high cost of peptide synthesis only a limited number of peptides are chemically made for further analysis. Here we describe an accurate and cost effective method to bin peptides on-phage based on binding region(s), without any requirement for peptide or protein synthesis. This approach, which integrates...
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