Article
CRISPR-Cas9 gene editing causes alternative splicing of the targeting mRNA.
Biochemical and biophysical research communications - 12 Jul 2020
Zhang Qian, Fu Yao, Thakur Chitra, Bi Zhuoyue, Wadgaonkar Priya, Qiu Yiran, Xu Liping, Rice M'Kya, Zhang Wenxuan, Almutairy Bandar, Chen Fei
Abstract excerpt
The technique of CRISPR-Cas9 gene editing has been widely used to specifically delete the selected target genes through generating double strand breaks (DSBs) and inducing insertion and/or deletion (indel) of the genomic DNAs in the cells. We recently applied this technique to disrupt mineral dust-induced gene (mdig), a potential oncogene as previously reported, by single guide RNA (sgRNA) targeting the third...
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