Article
Resistance-Conferring Mutations on Whole-Genome Sequencing of Fluoroquinolone-resistant and -Susceptible Mycobacterium tuberculosis Isolates: A Proposed Threshold for Identifying Resistance.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America - 1 Jun 2021
Maruri Fernanda, Guo Yan, Blackman Amondrea, van der Heijden Yuri F, Rebeiro Peter F, Sterling Timothy R
Abstract excerpt
BACKGROUND: Fluoroquinolone resistance in Mycobacterium tuberculosis (Mtb) is conferred by DNA gyrase mutations, but not all fluoroquinolone-resistant Mtb isolates have mutations detected. The optimal allele frequency threshold to identify resistance-conferring mutations by whole-genome sequencing is unknown. METHODS: Phenotypically ofloxacin-resistant and lineage-matched ofloxacin-susceptible Mtb isolates...
Topics
- Antitubercular Agents
- DNA Gyrase
- Drug Resistance, Bacterial
- Fluoroquinolones
- Microbial Sensitivity Tests
- Mutation
- Mycobacterium tuberculosis
- Ofloxacin