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Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?

28 Apr 2020

Abstract excerpt

BACKGROUND: High-throughput sequencing techniques are used to analyse the diversity of the respiratory microbiota in health and disease. Although extensive data are available regarding bacterial respiratory microbiota, its fungal component remains poorly studied. This is partly due to the technical issues associated with fungal metagenomics analyses. In this study, we compared two DNA extraction protocols and two...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 32343737
DOI: 10.1371/journal.pone.0232215

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