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Evaluation of V3–V4 and FL-16S rRNA amplicon sequencing approach for microbiota community analysis of tracheostomy aspirates

22 Jul 2025

Abstract excerpt

ABSTRACT Respiratory infections pose a significant risk for people requiring prolonged mechanical ventilation, yet limited information exists regarding the complex microbiome dynamics of people with tracheostomies during chronic critical illness. Oxford Nanopore Technologies (ONT) long-read sequencing allows for full-length 16S rRNA amplicon sequencing, providing enhanced species-level understanding of the...

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PMID: 40693760
DOI: 10.1128/msphere.00388-25

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