Article
A mutant of Pseudoalteromonas carrageenovora arylsulfatase with enhanced enzyme activity and its potential application in improvement of the agar quality.
Food chemistry - 1 Aug 2020
Zhu Yanbing, Liang Meifang, Li Hebin, Ni Hui, Li Lijun, Li Qingbiao, Jiang Zedong
Abstract excerpt
Enzymatic desulfation using arylsulfatase provides an attractive approach to improve agar quality. We have previously characterized a functional arylsulfatase from Pseudoalteromonas carrageenovora. To further improve its enzymatic performance, we isolated a mutant arylsulfatase of K253Q with improved enzyme activity from a random mutant library. Compared to wild-type arylsulfatase (WT), K253Q showed 33% increase...
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