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Article

Knockout of secondary alcohol dehydrogenase in Nocardia cholesterolicum NRRL 5767 by CRISPR/Cas9 genome editing technology.

PloS one - 1 Jan 2020

Huang Jenq-Kuen, Samassekou Kadidia, Alhmadi Hekmat B, VanDerway David R, Diaz Joshua D, Seiver Jacob A, McClenahan Shawn W, Holt Scott M, Wen Lisa

Abstract excerpt

Nocardia cholesterolicum NRRL 5767 is well-known for its ability to convert oleic acid to 10-hydroxystearic acid (~88%, w/w) and 10-ketostearic acid (~11%, w/w). Conversion of oleic acid to 10-hydroxystearic acid and then to 10-ketostearic acid has been proposed to be catalyzed by oleate hydratase and secondary alcohol dehydrogenase, respectively. Hydroxy fatty acids are value-added with many industrial...

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PMID: 32218601
DOI: 10.1371/journal.pone.0230915
PMCID: PMC7101164

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