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Article

Artificial control of the multistep oxidation reactions catalyzed by the cytochrome P450 enzyme RosC.

Applied microbiology and biotechnology - 1 Apr 2020

Iizaka Yohei, Kanai Hiroshi, Suzuki Tomoko, Maruyama Yuna, Kurita Misa, Sano Momoho, Watanabe Arisa, Fukumoto Atsushi, Saito Ryota, Anzai Yojiro

Abstract excerpt

The cytochrome P450 monooxygenase RosC catalyzes the three-step oxidation reactions, which leads to the formation of a hydroxy, formyl, and carboxy group at C-20 during rosamicin biosynthesis in Micromonospora rosaria IFO13697. To determine if amino acid substitutions in RosC could allow for the control of the multistep oxidation reactions, we screened RosC random mutants. The RosC mutant RM30, with five amino...

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Identifiers

PMID: 32103316
DOI: 10.1007/s00253-020-10481-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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