Article
Haplotyping by CRISPR-mediated DNA circularization (CRISPR-hapC) broadens allele-specific gene editing.
Nucleic acids research - 18 Mar 2020
Yu Jiaying, Xiang Xi, Huang Jinrong, Liang Xue, Pan Xiaoguang, Dong Zhanying, Petersen Trine Skov, Qu Kunli, Yang Ling, Zhao Xiaoying, Li Siyuan, Zheng Tianyu, Xu Zhe, Liu Chengxun, Han Peng, Xu Fengping, Yang Huanming, Liu Xin, Zhang Xiuqing, Bolund Lars, Luo Yonglun, Lin Lin
Abstract excerpt
Allele-specific protospacer adjacent motif (asPAM)-positioning SNPs and CRISPRs are valuable resources for gene therapy of dominant disorders. However, one technical hurdle is to identify the haplotype comprising the disease-causing allele and the distal asPAM SNPs. Here, we describe a novel CRISPR-based method (CRISPR-hapC) for haplotyping. Based on the generation (with a pair of CRISPRs) of extrachromosomal...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.