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Article

Selection is required for efficient Cas9-mediated genome editing in Fusarium graminearum.

Fungal biology - 1 Jan 2000

Gardiner Donald M, Kazan Kemal

Abstract excerpt

Genome engineering using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated nucleases, such as Cas9 (CRISPR-associated protein 9), are revolutionising molecular biology. In this study, we established a Cas9-based genome editing system in Fusarium graminearum, a highly destructive fungal pathogen of cereal crops. Although the molecular toolkit of F. graminearum is well developed compared...

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PMID: 29458716
DOI: 10.1016/j.funbio.2017.11.006

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