Article
Whole-exome sequencing reveals the impact of UVA light mutagenesis in xeroderma pigmentosum variant human cells.
Nucleic acids research - 28 Feb 2020
Moreno Natália Cestari, de Souza Tiago Antonio, Garcia Camila Carrião Machado, Ruiz Nathalia Quintero, Corradi Camila, Castro Ligia Pereira, Munford Veridiana, Ienne Susan, Alexandrov Ludmil B, Menck Carlos Frederico Martins
Abstract excerpt
UVA-induced mutagenesis was investigated in human pol eta-deficient (XP-V) cells through whole-exome sequencing. In UVA-irradiated cells, the increase in the mutation frequency in deficient cells included a remarkable contribution of C>T transitions, mainly at potential pyrimidine dimer sites. A strong contribution of C>A transversions, potentially due to oxidized bases, was also observed in non-irradiated XP-V...
Topics
- Cell Line
- DNA Damage
- DNA Repair
- DNA Replication
- Humans
- Mutagenesis
- Mutation
- Oxidative Stress
- Pyrimidine Dimers
- Ultraviolet Rays
- Exome Sequencing
- Xeroderma Pigmentosum