Article
Targeted Isolation and Characterization of T-DNA Mutants Defective in Photorespiration.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Timm Stefan, Modde Katharina, Bauwe Hermann
Abstract excerpt
Transfer DNA (T-DNA) insertional lines have facilitated reverse genetic approaches in plant science and considerably accelerated the functional characterization of genes. Typically, online databases of mutant collections are searched for suitable mutant alleles of the gene of interest (GOI). Before such lines can be characterized physiologically, the genotype of the respective mutant has to be verified followed...
Topics
- Arabidopsis
- Carbon Dioxide
- Chlorophyll
- Chlorophyll A
- DNA
- Fluorescence
- Gene Expression Regulation, Plant
- Genotype
- Mutagenesis, Insertional
- Optical Imaging
- Oxygen
- Oxygen Consumption
- Phenotype
- Photosynthesis
- Plant Leaves
- RNA, Messenger
- Ribulose-Bisphosphate Carboxylase
