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An anti-apoptotic HEK293 cell line provides a robust and high titer platform for transient protein expression in bioreactors

24 Mar 2019

Abstract excerpt

HEK293 transient expression systems are used to quickly generate proteins for research and pre-clinical studies. With the aim of engineering a high-producing host that grows and transfects robustly in bioreactors, we deleted the pro-apoptotic genes Bax and Bak in an HEK293 cell line. The HEK293 Bax Bak double knock-out (HEK293 DKO) cell line exhibited resistance to apoptosis and shear stress. HEK293 DKO cells...

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Identifiers

PMID: 30907238
DOI: 10.1080/19420862.2019.1598230

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