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Rapid identification of pathogens from positive blood culture bottles with the MinION nanopore sequencer

12 Oct 2018

Abstract excerpt

Purpose. Bloodstream infections are major causes of morbidity and mortality that lead to prolonged hospital stays and higher medical costs. In this study, we aimed to evaluate the MinION nanopore sequencer for the identification of the most dominant pathogens in positive blood culture bottles. Methodology. 16S and ITS1-5.8S-ITS2 rRNA genes were amplified by PCR reactions with barcoded primers using nine clinical...

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PMID: 30311873
DOI: 10.1099/jmm.0.000855

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