Article
Comprehensive antibiotic-linked mutation assessment by resistance mutation sequencing (RM-seq).
Genome medicine - 31 Aug 2018
Guérillot Romain, Li Lucy, Baines Sarah, Howden Brian, Schultz Mark B, Seemann Torsten, Monk Ian, Pidot Sacha J, Gao Wei, Giulieri Stefano, Gonçalves da Silva Anders, D'Agata Anthony, Tomita Takehiro, Peleg Anton Y, Stinear Timothy P, Howden Benjamin P
Abstract excerpt
Mutation acquisition is a major mechanism of bacterial antibiotic resistance that remains insufficiently characterised. Here we present RM-seq, a new amplicon-based deep sequencing workflow based on a molecular barcoding technique adapted from Low Error Amplicon sequencing (LEA-seq). RM-seq allows detection and functional assessment of mutational resistance at high throughput from mixed bacterial populations. The...
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