Article
In vivo analysis of outer arm dynein transport reveals cargo-specific intraflagellar transport properties.
Molecular biology of the cell - 15 Oct 2018
Dai Jin, Barbieri Francesco, Mitchell David R, Lechtreck Karl F
Abstract excerpt
Outer dynein arms (ODAs) are multiprotein complexes that drive flagellar beating. Based on genetic and biochemical analyses, ODAs preassemble in the cell body and then move into the flagellum by intraflagellar transport (IFT). To study ODA transport in vivo, we expressed the essential intermediate chain 2 tagged with mNeonGreen (IC2-NG) to rescue the corresponding Chlamydomonas reinhardtii mutant oda6. IC2-NG...
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