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One-pot two-strain system based on glucaric acid biosensor for rapid screening of myo-inositol oxygenase mutations and glucaric acid production in recombinant cells.

Metabolic engineering - 1 Sept 2018

Zheng Shuang, Hou Jin, Zhou Yi, Fang Hao, Wang Ting-Ting, Liu Fei, Wang Feng-Shan, Sheng Ju-Zheng

Abstract excerpt

The development of D-glucaric acid (GA) production in recombinant cells has leapt forward in recent years, and higher throughput screening and selection of better-performing recombinant cells or biocatalysts is in current demand. A biosensor system which converts GA concentration into fluorescence signal in Escherichia coli was developed in 2016, but its application has rarely been reported. Herein, an effective...

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PMID: 30125674
DOI: 10.1016/j.ymben.2018.08.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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