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Article

Multiplex mutagenesis of four clustered CrRLK1L with CRISPR/Cas9 exposes their growth regulatory roles in response to metal ions.

Scientific reports - 15 Aug 2018

Richter Julia, Watson James Matthew, Stasnik Peter, Borowska Monika, Neuhold Jana, Berger Matthias, Stolt-Bergner Peggy, Schoft Vera, Hauser Marie-Theres

Abstract excerpt

Resolving functions of closely linked genes is challenging or nearly impossible with classical genetic tools. Four members of the Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) family are clustered on Arabidopsis chromosome five. To resolve the potentially redundant functions of this subclass of CrRLK1Ls named MEDOS1 to 4 (MDS1 to 4), we generated a single CRISPR/Cas9 transformation vector using a...

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Identifiers

PMID: 30111865
DOI: 10.1038/s41598-018-30711-3
PMCID: PMC6093868

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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