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Helper-free Production of Laboratory Grade AAV and Purification by Iodixanol Density Gradient Centrifugation

7 May 2018

Abstract excerpt

Adeno-associated virus (AAV) is one of the most promising gene therapy vectors and is widely used as a gene delivery vehicle for basic research. As AAV continues to become the vector of choice, it is increasingly important for new researchers to have access to a simplified production and purification protocol for laboratory grade recombinant AAV. Here we report a detailed protocol for serotype independent...

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PMID: 30073177
DOI: 10.1016/j.omtm.2018.05.001

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